Table 1.

Nonenzymatic modification of nitrotyrosine

Sample composition	Amounts of detected nitrotyrosine, pmol
	37°C incubation	100°C incubation
Nitrotyrosine, 1 mM	132	164
Nitrotyrosine, 1 mM and DTT, 10 mM	128	148
Nitrotyrosine, 1 mM and spleen homogenates*	125	149
Nitrotyrosine, 1 mM, spleen homogenates and DTT, 10 mM	125	22
Nitrotyrosine, 1 mM and hemoglobin, 25 μM	136	147
Nitrotyrosine, 1 mM, hemoglobin, 25 μM, and DTT, 10 mM	85	<1

Nitrotyrosine (100 nmol) was incubated with 10 mM DTT and indicated amounts of tested compounds. An equivalent of 180 pmol of nitrotyrosine was applied on the HPLC and analyzed by ECD. 

*Protein (50 μg). Data from a representative experiment are presented.