Table 2.
Heme-dependent reduction of nitrotyrosine
| Heme protein or compound | Nitrotyrosine conversion at 100°C, % |
|---|---|
| Hemoglobin, 25 μM | 100% |
| 2.5 μM | 100% |
| 0.25 μM | 73% |
| Myoglobin, 25 μM | 100% |
| Cytochrome C, 25 μM | 100% |
| Hemin, 25 μM | 100% |
| 2.5 μM | 100% |
| 0.25 μM | 24% |
| Protoporphyrin IX, 25 μM | 0% |
| Protoporphyrin IX with 25 μM Fe2+ | 100% |
| Fe3+, 25 μM | 0% |
| Fe2+, 25 μM | 0% |
| Pyrrole, 25 μM with Fe2+, 25 μM | 16% |
| Vitamin B12, 25 μM | 57% |
Nitrotyrosine (100 nmol) was incubated with 10 mM DTT and indicated amounts of tested compounds. An equivalent of 180 pmol of nitrotyrosine was applied on the HPLC and analyzed by ECD. The percentage of conversion was calculated by the comparison with amount of detected nitrotryosine in the sample lacking DTT and hemoglobin. Data from a representative experiment are presented.