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Transcriptional activation at the glnA promoter by Switch I mutant forms of NtrC. Single-cycle transcription assays were performed as described in Materials and Methods. The template was supercoiled plasmid pJES534 (1 nM), which carries a strong enhancer ≈460 bp upstream of the glnA promoter (21). Relevant NtrC proteins are indicated next to the curves: WT, MBP-NtrCWT; A216C, MBP-NtrCA216C; G219C, MBP-NtrCG219C.
