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Transcriptional activation by MBP-NtrCWT (A) and MBP-NtrCA216C (B) on supercoiled templates carrying or lacking an enhancer. Single-cycle transcription assays were performed as described in Materials and Methods on supercoiled plasmid pJES534 (1 nM), which carries two strong NtrC-binding sites (+enhancer), or pJES535 (1 nM), which lacks specific NtrC-binding sites (−enhancer).
