Table 1.
Protein | Without enhancer DNA | With enhancer DNA† | With nonspecific DNA† |
---|---|---|---|
NtrCWT | 17 | 615 | 18 |
NtrCA216C | 320 | 505 | 290 |
NtrCG219C | 280 | 450 | 260 |
ATPase activity (pmol/60 min per 10 μl) was measured at 100 nM NtrC protein at 37°C. Proteins were phosphorylated as described in Materials and Methods.
†See Materials and Methods.