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Summary of ATPase parameters
| Motor | Condition | Km, μM tubulin | Vmax, ATP/s per head |
|---|---|---|---|
| One-headed | In solution | 1.8 ± 0.5 | 3.3 ± 0.2 |
| One-headed | 1 motor/bead | 50 ± 24 | 5.8 ± 1.9 |
| One-headed | 500 motors/bead | 0.29 ± 0.06 | 2.8 ± 0.2 |
| Two-headed | 1 motor/bead | 7.4 | 44 |
Shown are Michaelis-Menten parameters Vmax (maximum microtubule-stimulated ATPase rate) and Km (tubulin concentration, in polymerized form, for half-maximal ATPase rate). Two-headed data are from Coy et al. (13).
