Figure 2.

Expression of Eμ-c-myc transgene results in an increase in B cell size (growth) during all stages of B cell development. (A) Total bone marrow cells isolated from Eμ-c-myc transgenic or littermate control mice were stained with PE-conjugated anti-B220 and FITC-conjugated anti-IgM. Cells were visualized by flow cytometry. Shown are the FSC of gated B220^loIgM⁻ pro-B/pre-B cells, B220^loIgM⁺ immature B cells and B220^hiIgM⁺ mature B cells. Cells with higher FSC are larger than cells with lower FSC. (B) Total splenocytes were stained with FITC-conjugated anti-IgD and PE-conjugated anti-IgM. Shown are the FSC of IgM^hiIgD^lo immature B cells, IgM^hiIgD^hi, and IgM^loIgD^hi mature B cells as visualized by flow cytometry. (C) Purified splenic B lymphocytes from either Eμ-c-myc transgenic or littermate control mice were analyzed for cell volume utilizing a Coulter Z2 counter (47). Shown is a representative single-parameter histogram.