Figure 4.

Expression of Eμ-c-myc transgene results in increased protein synthesis. (A) Purified splenic B lymphocytes (2 × 10⁶ cells) isolated from Eμ-c-myc or littermate control mice were lysed in TNT buffer with protease inhibitors, and total protein was determined by the Bradford method. Error bars denote standard error for triplicate samples from a representative experiment. (B) Purified splenic B lymphocytes (2 × 10⁶ cells) from the above mice were cultured for 30 min in methionine-free medium, followed by 30 min in [³⁵S]methionine-containing medium. Cells were washed and lysed in RIPA buffer (48). Lysate was spotted onto glass filters, and [³⁵S]methionine incorporation was determined by using a liquid scintillation counter.