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. 1999 Nov 9;96(23):13186–13190. doi: 10.1073/pnas.96.23.13186

Figure 1.

Figure 1

Hematopoietic progenitor assays for BCR-ABL and v-SRC transduced EpoR−/− fetal liver cells. CFU-E (A) and BFU-E (B) colonies were generated in cultures of 105 EpoR−/− fetal liver cells infected with retroviral constructs encoding EpoR, BCR-ABL, and v-SRC. Total CFU-E and BFU-E were counted after 2 (A) and 8 days (B), respectively. Results are presented as percentage of EpoR rescued colonies in the optimum conditions (cultures containing Epo, IL-3, IL-6, and SF) shown by the hatched bars. Graphs are from duplicate cultures of 3 (v-SRC) to 10 (all other constructs) independent experiments. Under the optimum condition (cultures containing spleen conditioned media and Epo), the average of the absolute number of CFU-E colonies rescued by EpoR was 1,698 ± 426 (n = 4) per 106 cells plated. Similarly, the average of absolute number of BFU-E rescued by EpoR-infected cells under the optimum condition (Epo, IL-3, IL-6, and SF) was 68 ± 12 per 105 cells (n = 10).