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. 1999 Nov 9;96(23):13191–13196. doi: 10.1073/pnas.96.23.13191

Figure 2.

Figure 2

PARP and p53 expression in immortalized wild-type, PARP−/−, and PARP−/−(+PARP) fibroblasts. (A) Cell extracts of wild-type, PARP−/−, and PARP−/−(+PARP) fibroblasts (30 μg protein) were subjected to immunoblot analysis with antibodies to PARP (Top) and to PAR (Middle). RT-PCR was performed with specific human (hPARP) and mouse (mPARP) PARP primers (Bottom). (B) Cell extracts were subjected to immunoblot analysis with mAb to p53 (PAb421) (Top). The blot was stained with Ponceau S to verify equal loading and transfer of proteins in both lanes (Middle). RT-PCR and PCR were performed with specific primers for p53 mRNA and gene (Bottom). The positions of PARP, PAR, p53, and PARP and p53 cDNA are indicated.