Figure 5.
Location of Rb-1 and Jun in chromosomal regions with copy number changes in PARP−/− mice and altered expression of Rb and Jun in these animals. (A) CGH profile of chromosome 14 of the PARP−/− mice showing the loss of 14D3-ter, and the location of Rb-1 and Glud (arrows) on 14D3. Immunoblot analysis with antibodies to Rb and glutamate dehydrogenase (GDH) of tissue extracts from wild-type and PARP−/− mice. (B) CGH profile of chromosome 4 of PARP−/− mice showing the partial gain of 4C5-ter and the location of Jun (arrow) on mouse chromosome 4C5. The immunoblot in A was reprobed with antibodies to Jun. (C) Balanced CGH profile of chromosome 2 of PARP−/− mice and the location of Pcna and TopI genes (arrows) on 2B-C and 2H. The immunoblot in A was reprobed with antibodies to PCNA and to topo I. The positions of Rb (110 kDa), glutamate dehydrogenase (61 kDa), Jun (39 kDa), PCNA (36 kDa), and topo I (100 kDa) are indicated. (D) RT-PCR and PCR analysis of wild-type and PARP−/− mice liver using p53 and Rb-specific primers. The positions of p53 and Rb cDNA (arrows) and of DNA size standards (in kb and bp) are indicated.