Figure 3.

Immunoblotting of different rat tissues with anti-PV-1C polyclonal antibody. Total membrane proteins from adrenals (5 μg, lane A), intestinal mucosa (100 μg, lane I), kidney (100 μg, lane K), and pancreas (10 μg, lane P) were subjected to immunoblotting, and the signal was revealed by enhanced chemiluminescence. The reason for the uneven loading was the high level of expression of PV-1 in adrenals and pancreas on one side and the low level in intestinal mucosa and kidney on the other side; as determined by preliminary experiments, the signal in adrenal glands and pancreas would interfere with the signal in the other tissues when equal amounts of protein were loaded.