Table 2.

Rates of minichromosome loss in bir1Δ cells

Genotype	Red colonies	Half-sectored colonies*	Total colonies	Loss rate/cell division†	Fold increase‡
BIR1	6	6	5,409	1.11  ×  10−3	1.0
bir1Δ	134	27	4,878	5.69  ×  10−3	5.1

The colony color sectoring assay was performed with isogenic wild-type BIR1 (YHY401) and bir1Δ (YHY402) strains. A single colony from each strain was grown overnight in SD/His⁻ medium and plated for single colonies on yeast extract/peptone/dextrose. 

^*Includes colonies that contain at least a full half sector. The mother cell underwent a chromosome loss or missegregation event in the first division. 

^† Loss rate/cell division = half-sectored colonies/(total colonies − red colonies). 

^‡ Fold increase = loss rate/cell division of bir1Δ cells divided by loss rate/cell division of BIR1 cells.