TABLE 1.
Genes Evaluated by Northern Blot, qRT/PCR, and Microarray
| Gene Name | PCR Forward Primer* | PCR Reverse Primer* | PCR Product Length* |
|---|---|---|---|
| Vimentin | TTCTCAGCATCACGATGAC | TTGGTTGGATACTTGCTGG | 315 |
| laminin receptor 1 (LAMR1) | GTTTGATGTGGTGGATGCTG | CGCTCCAGTCTTCAGTAGGG | 291 |
| Macrophage migration inhibitory factor (MIF) | AGAACCGCTCCTACAGCAAG | GAGTTGTTCCAGCCCACATT | 123 |
| Eukaryotic translation initiation factor 3, subunit 3 (EIF3S3) | GTCGCCAGCAGGAGAATAT | AGAGTTGCCCTGGTGTGAC | 283 |
| NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 (NDUFB8) | TACAACAGGAACCGTGTGGA | CTGGTTCTTTGGAGGGATCA | 202 |
| Ubiquinol-cytochrome c reductase hinge protein (UQCRH) | ATGCGAGCAGTTGGAGAAAT | CTACCAGCCTCAAGCCAAAC | 310 |
| Thioredoxin-like (TXNL) | CTCCAGTCCAGGCAACAAAT | GCTTGGCAAAAGTGGTGACT | 269 |
| Proteasome (prosome, macropain) subunit, beta type, 4 (PSMB4) | GCTCGAAGATGAACCCTTTG | ACCTTTTTCGGTGACAGTGG | 290 |
| Ribosomal protein S20 (RPS20) | AAGGGCTGAGGATTTTTGGT | GGAAACGATCCCACGTCTTA | 341 |
| Syntaxin binding protein 2 (STXBP2) | GAGCCCACCTATCAGCTGTC | GCTTCTTGTCCAGTGCCTTC | 361 |
| Ribosomal protein, large, P0 (RPlP0) | TCGACAATGGCAGCATCTAC | CGACTCTTCCTTGGCTTCAA | 335 |
| Small nuclear ribonucleoprotein polypeptide F (SNRPF) | TGTCTGGACATCTGGGTGAA | TCCCCCACAAAAGATGCTAT | 114 |
| ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1 (ATP5A1) | CGTGCGTCTAACTGAGTTGC | AGCTTCAAATCCAGCCAAGA | 268 |
| S100 calcium-binding protein A13 (S100A13) | TAATGGCAGCAGAACCACTG | TTGAGCTCCGAGTCCTGATT | 217 |
| Cytochrome c oxidase subunit VIIb (COX7B) | AGCTGTATTGCCGCAGTTCT | TTTGGGGTAACTCTGCCAAC | 259 |
| B-cell associated protein (REA) | GATCGCCACATCACAGAATC | AGCAGATCCACTTCCTCTGG | 156 |
| proteasome (prosome, macropain) subunit, alpha type, 3 (PSMA3) | CAAGCTGCAAAGACGGAAAT | CCCAGCTGAGTTCTAGTTCAAAA | 139 |
| sapiens ring-box 1 (RBX1) | CAAGAAGCGCTTTGAAGTGA | TGGACACACCTGTCGTGTTT | 232 |
| Arsenate resistance protein ARS2 (ARS2) | GATCTTGGGCTATGGAGCTG | TTCCCTGTGGAAGTGCTTTT | 337 |
| Ribosomal protein L13a (RPl13A) | CTGGACCGTCTCAAGGTGTT | TATTGGGCTCAGACCAGGAG | 326 |
| Ribosomal protein S16 (RPS16) | GGCAATGGTCTCATCAAGGT | TCTCCTTCTTGGAAGCCTCA | 241 |
| 2,4-Dienoyl CoA reductase 1, mitochondrial (DECR1) | AACTCGCAAATCTTGCTGCT | TTCTATGGTGTCCCACTGCTC | 153 |
| Signal sequence receptor, delta (translocon-associated protein DELTA) (SSR4) | ACGTCGGTGGAAAACAATTC | GATGGTCCACGCTGACTGTA | 210 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | CCAGGTGGTCTCCTCTGACT | AGGGGTCTACATGGCAACTG | 303 |
| Gene Name | qPCR Forward Primer** | qPCR Reverse Primer** | qPCR Probe** |
| Vimentin | GGACACTTCTGATTAAGACGGTTGA | GTGTGCAATTTTTATTCAAGGTCATCGT | cAggTTATcAAcgAAAcTT |
| Laminin receptor 1 (LAMR1) | TGCCCTCTGTGCCTATTCAG | GGAGCTGCAGACCAGTCTTC | cAAttccctActGAAGActG |
| Macrophage migration inhibitory factor (MIF) | GCGCCTGCGCATCAG | CCGCGTTCATGTCGTAATAGTTGAT | cccGGAcAGGGtctAc |
| Eukaryotic translation initiation factor 3, subunit 3 (EIF3S3) | GATGGACTCGCTGCTCATTG | GGGCAGTGAACTCCTTGATGTT | cAGGccAGAtAAAcAc |
| NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8 (NDUFB8) | GGGACGTGTACCCTGTCTAC | CCTCGTTCCAGGTACAGATTATTGT | ctttGGtcccAcAGGctG |
| Ubiquinol-cytochrome c reductase hinge protein (UQCRH) | AGGAGCTCTTTGACTTCTTGCAT | GGTGCAACTTAAGTCCACACATTTAT | ccAttGcGtGGcccAc |
| Thioredoxin-like (TXNL) | TCCAGTCCAGGCAACAAATATGAAT | TGTCTTTTGTACCTTAGTGGCTTTCT | ccAAc TAcTcgTTTgAAgTc |
| Proteasome (prosome, macropain) subunit, beta type, 4 (PSMB4) | CTACAGAGACCAACTGGGATATTGC | GCAACTTCAGTTCTGGATAATGCA | cAcAtGAtcAGtGGctttG |
| Ribosomal protein S20 (RPS20) | GTGAAAGGACCAGTTCGAATGC | ACGTCTTAGAACC TTCACCACAAG | ccAAGActttGAGAAtcAc |
| Syntaxin binding protein 2 (STXBP2) | GCCACCGAGGGCAAGT | GCCTTCAGGTCATCCAGGAA | ctcAttGGctcctcAcAcAt |
| Ribosomal protein, large, P0 (RPLP0) | GGATTACACC TTCCCACTTGCT | GCCACAAAGGCAGATGGATCA | AAGGccttGAccttttc |
| Small nuclear ribonucleoprotein polypeptide F (SNRPF) | ATGGAGCTTTGTCTGGACATCTG | TTCCTCTTCTTCCACACCTCTGATA | AcAttAttAcAccttAttAAAActtc |
| ATP synthase, H+ transporting, mitochondrial F1 COMPLEX, ALPHA SUBUNIT, ISOFORM 1 (ATP5A1) | CCAGCACCAAGCCTTGTTG | AGCTTTGCATCTGATTGTTCTGAGA | cAtcAGccctGAtAGtGc |
| S100 calcium-binding protein A13 (S100A13) | CCAGCAGTTGCCCCATCT | CACATCCAAGCTCTTCATCTTCTCA | AAGGAtGtGGGctctctt |
| Cytochrome c oxidase subunit VIIb (COX7B) | TGGAGCCACTTTCTGTATTGTTACA | CAGCTGGGATGATTACTGATTCCT | cttGtGttGctAcAtAtGtc |
| B-cell associated protein (REA) | CTTGTGCTGAACCTACAGGATGAA | TCTTACCCTTGATGAGGCTGTCA | cttcccctGGtGAAAct |
| Proteasome (prosome, macropain) subunit, alpha type, 3 (PSMA3) | GATCCATTAGGGTTACCAGACAGG | ACTTGTTGTGGCTGCTTGGA | cAtAGAtGAcAtGcctAtAAAA |
| Sapiens ring-box 1 (RBX1) | CATTGGACAACAGAGAGTGGGAATT | CAAAACAATTAAGCTTGATGGAAGAAGTCT | ctAGtGcccAtActtttG |
| Arsenate resistance protein ARS2 (ARS2) | AGGCCAGGGAGGTTATCCT | AGGTCCCGATATTCCACAATGG | tcGcAAcAGGAtGGttc |
| Ribosomal protein L13a (RPL13A) | CAAGATCCACTACCGGAAGAAGAAA | ACGTTCTTCTCGGCCTGTTT | ccGtAGcctcAtGAGctG |
| Ribosomal protein S16 (RPS16) | GCCCTGGTGGCCTATTACC | GGATGTCTTTGATCTCCTTC TTGGAA | cctcAtccAcAtAtttc |
| 2,4-dienoyl CoA reductase 1, mitochondrial (DECR1) | TGACGGTGGAGAGGAAGTACTTATT | TGTCTTCCTGATGAGTTCTTCTATGGT | tccttGGtGAcctttctc |
| Signal sequence receptor, delta (translocon-associated protein DELTA) (SSR4) | CCCGCCTCTGTTTACAGTCA | GCAGCCAGCACCTCAGT | AAGtGccccGAtGGtc |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | CGACCACTTTGTCAAGCTCATTTC | CCACCACCCTGTTGCTGTAG | cTggTATgAcAAcgAATTT |