TABLE 1.
Summary of Previous ABRF Edman Sequencing Studies
| Sample | Description | Study Purpose | Amount (pmol) | Positive Accuracy | Tentative Accuracy | No. of Participants |
|---|---|---|---|---|---|---|
| STD-1 | Peptide | Sequence analysis | 100 | 95% | 48% | 54 |
| ABRF-89SEQ | 2 peptides | Differentiation of 2 sequences | 240/48 | 95% | 57% | 48 |
| ABRF-90SEQ | Peptide conjugated to acetylated protein (PVDF) | Evaluate PVDF-bound sample | 30 | 82% | 43% | 55 |
| ABRF-91SEQ | Peptide conjugated to acetylated protein | Compare solution sample to PVDF | 50 | 83% | 55% | 90 |
| ABRF-92SEQ | Peptide | Determine 2 posttranslational modifications (PSer & HyPro) | 500 | 94% | 64% | 74 |
| ABRF-93SEQ | Rearrangement of STD1 | Determine improvement | 50 | 91% | 56% | 80 |
| ABRF 94SEQ | Protein | Cys & Trp determination | 50 | 96% | 55% | 78 |
| ABRF-95SEQ | Protein | 4 Cycles of microheterogeniety, length of sequence read | 45 | 78% | 45% | 71 |
| ABRF-96SEQA | Dataset | Single sequence calling ability | 40 | 100% | 86% | 95 |
| ABRF-96SEQB major | Dataset | Sequence calling ability in a mixture | 10 | 96% | 58% | 95 |
| ABRF-96SEQB minor | Dataset | Sequence calling ability in a mixture | 2 | 86% | 38% | 95 |
| ABRF-97SEQ major | Peptide, rear-rangement of 96SEQB major | Compare lab-generated data to previous year’s dataset | 10 | 92% | 54% | 50 |
| ABRF-97SEQ minor | Peptide, rear-rangement of 96SEQB minor | Compare lab-generated data to previous year’s dataset | 2 | 72% | 38% | 50 |
| ABRF-98SEQ | Peptide | Read low-level sequence & use MS/MS data if possible | 2.8 | 91% | 45% | 56 |
| ABRF-99SEQ protein | Protein/peptide mix | Distinguish 2 sequences, protein ID using BLAST search | 10 | 99% | 58% | 45 |
| ABRF-99SEQ peptide | Protein/peptide mix | Distinguish 2 sequences | 5 | 95% | 62% | 45 |
| ABRF-00SEQ | Rearrangement of ABRF-92SEQ | Determine 2 post translational modifications | 5 | 86% | 47% | 46 |
| ESRG-2002 | Protein | Heterogeneous N-terminus, protein ID using BLAST | 35 | 76% | 73% | 31 |
| ESRG-2003 | Protein | Homogeneous N-terminus, protein ID | 13.5 | 97% | 77% | 46 |
| ESRG-2004 | Peptide | Identify 7 post translational modifications | 775 | 96% | 57% | 20 |
| ESRG-2005 | Peptide | Identify 7 additional post translational modifications | 2000 | 80% | 42% | 27 |
| ESRG-2006 | 3 Peptide Mix | Sequence & quantify peptides identify modified amino acid | 40/40 (2 homologs) | 88% | 67% | 18 |
| 20 (third peptide) | 93% | 44% |