TABLE 2.
Amino Acid Analysis of the Peptide Mixture Used for the ESRG06 Study
| Amino Acid | Yield (nmol) | Corrected for 85/100 μL Injection | Peptide B Comp | Peptide B Expected nmol Yield | Peptide C Comp | Peptide C Expected nmol Yield | Total Expected nmol Yield | % Recovery |
|---|---|---|---|---|---|---|---|---|
| Asx | 0.3197 | 0.3761 | 2 | 0.4 | 0 | 0 | 0.4 | 94.02% |
| Thr | 0.1598 | 0.1880 | 1 | 0.2 | 0 | 0 | 0.2 | 94.00% |
| Ser | 0.8064 | 0.9488 | 1 | 0.2 | 1 | 0.8 | 1 | 94.88% |
| Glx | 1.1864 | 1.3958 | 4 | 0.8 | 1 | 0.8 | 1.6 | 87.24% |
| Pro | 0.0980 | 0.1153 | 1 | 0.2 | 0 | 0 | 0.2 | 57.65% |
| Gly | 0.8926 | 1.0502 | 1 | 0.2 | 1 | 0.8 | 1 | 105.02% |
| Ala | 1.1725 | 1.3794 | 4 | 0.8 | 1 | 0.8 | 1.6 | 86.21% |
| Cys | 0.0000 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| Val | 0.7115 | 0.8371 | 1 | 0.2 | 1 | 0.8 | 1 | 83.71% |
| Met | 0.0000 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| Ile | 0.1545 | 0.1817 | 1 | 0.2 | 0 | 0 | 0.2 | 90.85% |
| Leu | 1.7220 | 2.0259 | 4 | 0.8 | 2 | 1.6 | 2.4 | 84.41% |
| Tyr | 0.8451 | 0.9943 | 2 | 0.4 | 1 | 0.8 | 1.2 | 82.86% |
| Phe | 0.0000 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| Lys | 0.8644 | 1.0170 | 2 | 0.4 | 1 | 0.8 | 1.2 | 84.75% |
| His | 0.0154 | 0.0181 | 0 | 0 | 0 | 0 | 0 | - |
| Arg | 1.1850 | 1.3942 | 2 | 0.4 | 2 | 1.6 | 2 | 69.71% |
| Trp | N.D. | N .D. | 0 | 0 | 0 | 0 | 0 | - |
| NorVal | 1.9718 | 2.3198 | 92.79% | |||||
| Ave. | 85.79% | |||||||
| NorVal cor. | 92.46% |
This analysis was performed on 100 μL of the peptide mixture, expected to contain 400 pmol each of peptides C and C* and 200 pmol of peptide B. N.D., not detected.