Table 1.
5′UTR* | ORF region† | 144-bp portion‡ | 3′ UTR§ | |
---|---|---|---|---|
Kn | 0.339 ± 0.021 | 0.197 ± 0.020 | 0.187 ± 0.042¶ | 0.362 ± 0.044 |
Ks | 0.341 ± 0.058 | 0.267 ± 0.100 | ||
Ka | 0.153 ± 0.019 | 0.128 ± 0.038 | ||
Ka/Ks | 0.449 | 0.479 |
5′ untranslated region between rolB and rolC homologues (1,207 bp).
ORF region of NgrolB and RirolB gene (633 bp).
3′ untranslated region of NgrolB gene that corresponds to 3′ coding sequence of RirolB gene (144 bp).
3′ untranslated region from the site of RirolB termination codon to the site of the end of the left arm of the cellular T-DNA (318 bp; ref. 11)
Kn is significantly higher in the 5′ and 3′ UTRs than in the 144-bp portion, but is not significantly different between the ORF and 144 regions (P < 0.01; t-test).