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Bulletin of the World Health Organization logoLink to Bulletin of the World Health Organization
. 1991;69(3):331–333.

How long after being collected can blood still be cultured for chromosomal studies in the tropics.

R Elango 1, S Mathew 1, I C Verma 1
PMCID: PMC2393098  PMID: 1893508

Abstract

To examine how long after their collection samples of peripheral blood could be successfully cultured for cytogenetic analysis in tropical countries, we determined the mitotic index in cultures of blood stored for up to 7 days in summer and winter. The results indicate that chromosomal analysis is successful if blood is stored for up to 2 days in summer or for up to 4 days in winter before initiating culture. Storing the blood in tissue culture medium rather than in heparin solution did not significantly improve the mitotic index.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Martin P. K., Rowley J. D. The effect of refrigeration of bone marrow and peripheral blood on cytogenetic analysis. In Vitro Cell Dev Biol. 1986 Jul;22(7):387–391. doi: 10.1007/BF02623527. [DOI] [PubMed] [Google Scholar]
  2. Sharma T., Das B. C. The effect of storage of blood on the yield of X-ray-induced chromosome aberrations and spontaneous sister chromatid exchanges. Int J Radiat Biol Relat Stud Phys Chem Med. 1984 Feb;45(2):151–158. doi: 10.1080/09553008414550171. [DOI] [PubMed] [Google Scholar]

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