Abstract
To examine how long after their collection samples of peripheral blood could be successfully cultured for cytogenetic analysis in tropical countries, we determined the mitotic index in cultures of blood stored for up to 7 days in summer and winter. The results indicate that chromosomal analysis is successful if blood is stored for up to 2 days in summer or for up to 4 days in winter before initiating culture. Storing the blood in tissue culture medium rather than in heparin solution did not significantly improve the mitotic index.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Martin P. K., Rowley J. D. The effect of refrigeration of bone marrow and peripheral blood on cytogenetic analysis. In Vitro Cell Dev Biol. 1986 Jul;22(7):387–391. doi: 10.1007/BF02623527. [DOI] [PubMed] [Google Scholar]
- Sharma T., Das B. C. The effect of storage of blood on the yield of X-ray-induced chromosome aberrations and spontaneous sister chromatid exchanges. Int J Radiat Biol Relat Stud Phys Chem Med. 1984 Feb;45(2):151–158. doi: 10.1080/09553008414550171. [DOI] [PubMed] [Google Scholar]
