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. Author manuscript; available in PMC: 2009 May 30.
Published in final edited form as: J Neurosci Methods. 2008 Jan 20;170(2):181–187. doi: 10.1016/j.jneumeth.2008.01.009

Fig. 4.

Fig. 4

NbActiv4 increases total GABA puncta 40% and bright NR1 puncta 100% compared to neurons cultured in Neurobasal/B27. (A) and (B) Immunoreactive red GABA and green NR1 synaptic puncta. (C) and (D) Bright and dim segmentation thresholds for GABA and NR1 show effect of medium on stained puncta (n = 16 fields of 0.016 mm2 from 2 cultures).