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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Biomaterials. 2008 Apr 2;29(17):2680–2688. doi: 10.1016/j.biomaterials.2008.03.009

Figure 5. Effect of Bcl-2 levels on transfection activity of mRNA complexes.

Figure 5

(a) Luciferase expression. Cells were transfected for 4 h with 1 μg Luc-mRNA complexed with PLL or rPLL (N:P 2) to which DOTAP was added to form ternary complexes. Luciferase expression was measured after 6 h as mean RLU/mg cellular protein (n=3). Redox/Non-redox is the ratio (± SD) of transfection activity of rPLL-to-PLL complexes. Secondary axis (●) shows transfection in RLU/mg cellular protein mediated by rPLL complexes in the absence of serum. (b) eGFP expression. Cells were transfected with eGFP mRNA as described above and the gene expression was measured 24 h after transfection as mean % GFP positive cells (n=3). Redox/Non-redox is the ratio (± SD) of transfection activity of rPLL-to-PLL complexes. Secondary axis (●) shows % of GFP positive cells mediated by rPLL complexes in the absence of serum.