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. 1999 Nov 9;96(23):13264–13269. doi: 10.1073/pnas.96.23.13264

Figure 4.

Figure 4

Northern blot analysis confirms array data. (A) Induction of Six1, Myogenin, MyoD, Igfbp5, Igf2, and Slug by PAX3-FKHR transduction is demonstrated, as is the failure to induce these genes by PAX3. Igf2 expression appears by Northern blotting to be suppressed by PAX3. Basal c-Met levels in NIH 3T3 cells transduced by the empty virus is high and remains high in the Pax3 and PAX3-FKHR transduced clones. Pdgfra expression is repressed in the clones PF-1, PF-3, PAX3-C, and PAX3–1. (B) Myogenin protein expression is confirmed in the PAX3-FKHR transduced clones PF-C, PF-1, and PF-3 by Western analysis. (C) Northern blot analysis reveals expression of HSIX1, MYOG, IGFBP5, IGF2, Slug, and MERTK in several of the ARMS cell lines (RH3, RH4, RH5, RH28, and RH30). The first lane is normal muscle. A204 is an embryonal rhabdomyosarcoma that lacks the PAX3-FKHR chimeric oncogene.