Table 1.
Muscle-related genes induced by PAX3-FKHR transduction
| Gene symbol | PF-C | PF-1 | PF-3 | PAX3-C | PAX3-1 | |
|---|---|---|---|---|---|---|
| Transcription factors | ||||||
| Myogenin | Myog | 14 | 58.9 | 22.3 | NS | NS |
| Six1 | Six1 | 5.3 | 4.7 | 3.5 | 0.7 | 0.8 |
| Slug | Slug | 2.6 | 2.4 | 2.5 | 0.8 | 0.9 |
| Signal transduction/growth factors | ||||||
| Insulin-like growth factor binding protein 5 | Igfbp5 | >100 | >100 | 91.2 | NS | NS |
| Insulin-like growth factor 2 | Igf2 | 7.9 | 17.6 | 3.1 | 0.3 | NS |
| Transforming growth factor β2 | Tgfβ2 | 2.8 | 3.8 | 0.9 | NS | NS |
| Contractility/Cell structure | ||||||
| Cardiac troponin T | Tnnt2 | 49.1 | 65.9 | 23.9 | NS | NS |
| Fast skeletal muscle troponin 1 | Tnni2 | 41.8 | >100 | 34.3 | NS | NS |
| Fast skeletal troponin C | Tnnc2 | 22.4 | 81.0 | 27.7 | NS | NS |
| Skeletal muscle myosin light chain 1 isoform | Myl1 | 16.9 | 80.4 | 0.7 | NS | NS |
| Perinatal skeletal myosin heavy chain | Myh8 | 10.2 | 76.1 | 7.9 | NS | NS |
| Atrial/fetal myosin alkali light chain | Myl4 | 4.3 | 60.7 | 11.7 | NS | NS |
| Alpha cardiac actin | Actc | 2.9 | 7.0 | 2.1 | 1.9 | 1.3 |
| Beta-tubulin gene M-β-2 | Tubb2 | 1.8 | 2.1 | 2.5 | 1.3 | 0.7 |
| Myosin binding protein H | Mybph | 1.8 | 7.8 | 1.8 | NS | NS |
| Skeletal muscle α actin | Acta1 | 1.8 | 7.4 | 1.4 | NS | NS |
| Tropomyosin isoform 2 | Tmp2 | 1.6 | 4.2 | 0.8 | 0.8 | 1.2 |
| Energy metabolism | ||||||
| Creatinine kinase M chain | CkM | 5.2 | 27.6 | 8.5 | NS | NS |
| Acyl-CoA dehydrogenase short-chain | Acads | 1.5 | 1.5 | 1.5 | 0.6 | 0.9 |
| Fructose-biphosphate aldolase A | AldoA | 1.2 | 2.0 | 2.1 | 1.4 | 2.0 |
| Synaptic transmission/electrochemical function | ||||||
| Acetylcholine receptor, γ-chain | Achrg | 17.5 | 68.8 | NS | NS | NS |
| Acetylcholine receptor α-subunit | Achra | NS | 17.3 | 7.9 | NS | NS |
| Ryanodine receptor, type 1 | Ryr1 | 3.4 | 19.8 | NS | NS | NS |
| Na+/H+-exchanger regulatory factor | Nherf | 2.9 | 3.4 | 2.7 | 2.1 | 3.2 |
| Na+/K+-transporting ATPase β-chain | Atp1b1 | 2.1 | 1.8 | 3.4 | 0.8 | 0.6 |
| Other | ||||||
| Complement factor H | Hf1 | 37.0 | 18.9 | 87.3 | NS | NS |
| H19 | H19 | 18.2 | 36.5 | NS | NS | NS |
| Clusterin | Clu | 1.6 | 0.3 | 2.0 | NS | NS |
Listed are genes with ≥50% induction (ratio of ≥1.5) by PAX3-FKHR transduction in at least two of the PF-C, PF-1, and PF-3 clones. A filter was used to exclude all spots with the maximum intensity <1,400 (on a scale of 0–65,535 fluorescent units) for either fluorochrome (designated NS). Of the 111 genes retrieved by this enquiry, 28 were found to be muscle-related as determined by a search of Medline database. Shown here are the gene name, gene symbol, functional categories, and the induction ratio for each of the experiments.