Figure 3.

Horizontal (A and B) and coronal (C and D) sections of transgenic embryos showing blue-stained regions in the E11.5 (A) and E12.5 (B) spinal cord, the forebrain region (C), and the eye (D). (A and B) β-Galactosidase-positive cells are located in the dorsolateral half of the spinal cord. Fainter staining is also noted ventrally. (C) Blue-stained cells are mainly confined to the subventricular zone of the lateral ganglionic eminence (LGE) in the forebrain. MGE, medial ganglionic eminence. (D) In the eye, X-Gal-positive cells are present dorsally in the inner layer of the optic cup. (Bars in A–C and D = 100 and 50 μm, respectively.)