Fig. 4.

mdx mice treated with AAV1-FS show decreased markers of muscle damage and aged mdx mice are responsive to FS treatment with functional benefit. (a) Serum creatine kinase levels (units/liter) are decreased at 3 months after injection with AAV1-FS compared with AAV1-GFP-injected controls. (*, P < 0.05; n = 10.) Error bars represent standard errors. (b) Hindlimb grip strength is significantly increased (P ≤ 0.05) at 275 days and beyond in aged mdx mice treated with AAV1-FS at 210 days of age (n = 15). Red, high-dose AAV1-FS; green, AAV1-GFP controls. (c) H&E stain of aged gastrocnemius demonstrates reduced pathology when injected at 210 days of age with FS compared with GFP-injected controls. (Original magnification, ×40.) (d) H&E stained diaphragm of animals injected at 210 days of age with FS shows less fat replacement than GFP-injected controls at late stage. (Original magnification, ×20.)