Fig. 3.

Effects of F427 mutations on PA insertion into liposomal membranes as measured by NBD fluorescence. (A) Representative recordings of NBD emission at 544 nm are shown upon PA pore insertion as a function of time. (B) Initial rates of change of NBD emission intensity were calculated from three independent experiments, as shown in A, with the same double exponential equation used for analyzing the data from K⁺ release. The rates were expressed as fractions of PA-WT. *, not detectable.