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. 2008 Mar 12;105(11):4312–4317. doi: 10.1073/pnas.0707370105

Fig. 5.

Fig. 5.

Potassium efflux is required for caspase-1 activation by LT, but is dispensable for pyroptosis induced by Salmonella. Macrophages were treated with LT or S. typhimurium (S.T) in standard medium containing high Na+ or modified medium containing Na+ replaced by K+. (A) Macrophages containing active caspase-1 were identified by FAM-YVAD staining; means ± SD are shown. *, P < 0.05 versus Na+ medium. (B) Mature IL-18 was detected in supernatants by Western blot. (C) Cleaved MEK3 (arrow) in LT-intoxicated macrophages was detected as in Fig. 3.