Fig. 1.

The generation of Mlh1^G67R mutant mice. (a) Domain structure of Mlh1. The location of the G67R mutation is indicated. (b) Schematic representation of the modified Mlh1^G67R genomic locus and sequence characteristics of the G67R mutation. (c) PCR genotyping of tail DNA from Mlh1^G67R mutant mice. Mlh1^G67R/+, heterozygous mice; Mlh1^G67R/G67R, homozygous mutant mice. After EcoRI restriction digestion of the PCR product, the WT allele is indicated by an 880-bp fragment and the Mlh1^G67R mutant allele by 470- and 410-bp restriction fragments. (d) Western blot analyses of thymocyte cell extracts using anti-Mlh1 and anti-Msh2 antibodies.*, Unspecific protein recognized by the Mlh1 antisera.