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. 2008 Mar 10;105(11):4168–4172. doi: 10.1073/pnas.0710285105

Fig. 3.

Fig. 3.

mSin3B is dispensable for cellular proliferation but required for cell cycle exit in vitro. (a) (Upper) Western blot analysis of mSin3B wild-type (lanes 1, 3, 5) and null (lanes 2, 4, 6) primary fibroblasts with the indicated antibodies. (Lower) Growth curves from mSin3B wild-type (n = 3) and null (n = 3) primary fibroblasts at passage 4. (b) BrdU incorporation after a 2-h pulse of 20 μM BrdU by mSin3B wild-type (white bars) or null (black bars) early-passage primary MEFs in 10% serum (Left) or 0.1% serum (Right). At least 200 cells were counted per point. Error bars indicate standard deviations. (c) Western blot of extracts from mSin3B wild-type or null MEFs infected or not with p16 using the indicated antibodies. (d) Proliferation of mSin3B wild-type (n = 3) or null (n = 3) MEFs after p16 or vector (V) infection, assayed by BrdU incorporation. At least 100 cells were counted for each point. (e) Growth curve of mSin3B wild-type (Left) or null (Right) MEFs after p16 or vector infection.