Fig. 2.

Detection of germ-line recombination. (a) Diagram illustrating progeny genotypes resulting from the mating between CD2/CD5;Pgk-Cre and CD2/CD5 mice. The CD2 and CD5 alleles are highlighted by dark and light ink fill, respectively. The proximal sequence (p) and distal sequence (d) flanking the loxP sites are highlighted by solid and stippled patterns, respectively. (b) PCR analysis of germ-line recombination between the CD2 and CD5 alleles. Tail samples were from 12 progeny of a cross between CD2/CD5;Pgk-Cre and CD2/CD5 mice. Positions of PCR primers are shown in Fig. 1a. The size of each PCR product is indicated on the right. M, DNA size markers.