Fig. 4.

P-Rex1 is widely distributed within the brain. (A) P-Rex1 Western blot with 6F12 mAb of P-Rex1 expression in adult mouse organs and purified BM-derived neutrophils from P-Rex1^+/+ (Wt) and P-Rex1^−/− (Ko) mice. Equal amounts of total lysate protein were loaded. (B) Western blot of P-Rex1 distribution in brain sections from adult Wt mice and deletion in P-Rex1^−/− (Ko) mice; 500 μg of tissue loaded per lane. (C) In situ hybridization of adult P-Rex1^−/− (Ko) and P-Rex1^+/+ (Wt) mouse brain slices by using a probe against exon 5 of mouse P-Rex1. (Scale bars, 2 mm.)