Table 1.

Characteristics of primers for sequencing BAC/PAC-nested deletions

Sequencing primer	Distance from Tn end, bases	Sequence read length, bases called by PHRED (trim mode)	BAC/PAC deletion template size, kb
Seq 1 5′-GACAAGATGTGTATCCACCTTAAC-3′	38	503	84
		497	65
		321	106
Seq 4 5′-CTCCCAGAGCCTGATAAAAACGG-3′	87	552	42
		528	66
		353	106
Seq 6 5′-CCGTGGAGGTAATAATTGACGATATG-3′	124	534	62
		458	70
		241	106
Seq 9 5′-GGTTCAGGGCAGGGTCGTTAAATAGC-3′	240	521	84
		408	58
		272	106
SP6II 5′-TTACGCCAAGCTATTTAGGTGACACTATAGAATAC-3′	N/A	544	54
		518	46
		312	106
T7L 5′-ATTGTAATACGACTCACTATAGGG-3′	N/A	540	90
		438	72
		415	61