Fig. 1.

BBS mice possess neuronal primary cilia in the brain but lack Sstr3-positive cilia. (A–C) Representative images of the CA3 region of the hippocampus in adult WT (A), Bbs2^−/− (B), and Bbs4^−/− (C) mice (n = 9) showing labeling for ACIII (red). Nuclei are stained with DRAQ5 (blue). The appearance and distribution of ACIII-positive cilia are similar among all genotypes. (D–F) The identical fields showing labeling for Sstr3 (green) reveals Sstr3-positive cilia in the WT (D) section but a complete lack of Sstr3-positive cilia in the Bbs2^−/− (E) or Bbs4^−/− (F) sections. (G–I) The merged images showing colocalization of ACIII and Sstr3 to cilia in the WT (G) section and no Sstr3 labeling of cilia in the Bbs2^−/− (H) or Bbs4^−/− (I) sections. (Scale bars, 10 μm.)