Fig. 3.

BBS mice lack Mchr1 ciliary labeling in the brain. (A–C) Representative images of the nucleus accumbens in adult WT (A), Bbs2^−/− (B), and Bbs4^−/− (C) mice (n = 9) showing labeling for ACIII (red). The appearance and distribution of ACIII-positive cilia are similar among all genotypes. (D–F) The identical fields showing labeling for Mchr1 (green) reveals Mchr1-positive cilia in the WT (D) section but a complete lack of Mchr1-positive cilia in the Bbs2^−/− (E) or Bbs4^−/− (F) sections. Note the presence of increased punctate labeling in the Bbs2^−/− (E) and Bbs4^−/− (F) sections. (G–I) The merged images showing colocalization of ACIII and Mchr1 to cilia in the WT (G) section and no Mchr1 labeling of cilia in the Bbs2^−/− (H) or Bbs4^−/− (I) sections. Nuclei are stained with DRAQ5 (blue). (Scale bars, 10 μm.)