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. 2008 Mar 11;105(11):4352–4357. doi: 10.1073/pnas.0712276105

Fig. 5.

Fig. 5.

The p72 RNA helicase does not directly interact with the exosome. (A and B) The 293TRex cells expressing myc-tagged p72 or myc-tagged ZAP were treated with tetracycline to induce ZAP or p72 expression and lysed in the lysis buffer in the presence (+) or absence (−) of 50 μg/ml RNase A. The proteins were immunoprecipitated with anti-hRrp46p (A) or anti-hRrp40p (B) antibody and Western blotted with the indicated antibodies. Input, total cell lysate; Ctrl, 293TRex cells; p72, 293TRex cells expressing p72; ZAP, 293TRex cells expressing ZAP. (C) The HEK293T cells expressing myc-tagged p72, myc-tagged ZAP, or both were lysed in the lysis buffer in the presence of 50 μg/ml RNase A. The proteins were immunoprecipitated with anti-hRrp40p antibody and Western blotted with the anti-myc antibody (Upper) or with the anti-hRrp40p antibody (Lower).