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. 2008 Mar 12;105(11):4489–4494. doi: 10.1073/pnas.0711414105

Fig. 4.

Fig. 4.

Effect of actin stabilization on endocytosis, plant development, asymmetric auxin distribution, and auxin efflux. (A and B) Endocytosis of FM4-64 (2 μM, 30 min). Pretreatment and cotreatment with solvent (1 h, A) and jasplakinolide (10 μM, 1 h, B). (Scale bars: 5 μm.) (C and D) Seedling development when germinated on plates containing TIBA (25 μM, C) and jasplakinolide (10 μM, D). (E–G) Auxin translocation as monitored by auxin-responsive DR5rev::GFP expression after 4-h gravistimulation on plates containing solvent (E), TIBA (F), and jasplakinolide (G). (H) Effects of TIBA and jasplakinolide on cellular efflux of auxin (NAA) and benzoic acid (BeA) from Arabidopsis cultured cells, shown in the 30 min after addition of radiolabeled NAA or BeA. (I and J) Equivalent presence of PM-localized PIN1-GFP in control (I) and TIBA-treated (10 μM for 30 min) (J) tobacco BY-2 cells. (Scale bars: 10 μm.) Note that under the same conditions, TIBA effectively inhibits auxin efflux in Arabidopsis cells.