Table 1.
Results of the biochemical analyses of the iipopeptide fraction of B. subtllis strain ATCC6633, determined as described in Material and Methods
| Surfactin | Myocosubtilin | |
|---|---|---|
| TLC | RfA = 0.90 | RfA = 0.23 |
| RfB = 0.65 | RfB = 0.27 | |
| RfC = 0.80 | RfC = 0.51 | |
| Durrum analyzer | Asx (1.0) Glx (1.0) | Asx (3.2) Glx (1.0) |
| Val (1.1) | Ser (0.8) Pro (1.0) | |
| Leu (3.2) | Tyr (0.8) | |
| Reverse-phase HPLC | Asx (1L) Glx (1L) | Asx: (1D, 2L) Glx (1L) |
| Val (1L) | Ser (0.8) Pro (1.0) | |
| HPLC | Leu (2L, 2D) | Tyr (1D) |
In the first row, the retention factors (Rf) for the three eluent systems used (A, B, and C) are given. The second row gives the results of the amino acid composition of the two lipopeptides determined with the Durrum analyzer. The thrid row gives the results of the absolute configuration determined with reverse-phase HPLC after derivatization with Marfey’s reagents.