Figure 3. The ability of proteolipid fusion proteins to complement the vma⁻ phenotype in proteolipid deletion strains.

Serial dilutions of yeast strains carrying plasmid-borne proteolipids and proteolipid fusions were spotted onto YEPD agar plates at either pH 5.5 or pH 7.5 and grown overnight at 30°C. Five μl of yeast culture grown to an absorbance at 600 nm of 0.20 was spotted onto the plate at the far left hand position for each pH and the spots to the right of this position correspond to five μl of serial 10-fold dilutions of the original culture.