Table 1. Amount of BRCA1 and BRCA2 proteins expressed by MCF-7, MDA-MB 231 and HBL 100 cells after treatment with 30 μM resveratrol and 48 h exposure.

		Proteins assayed (%)
	Cell lines	BRCA1	BRCA2
MCF-7	Untreated controls	0.58±0.01	1.00±0.03
	30 μM resveratrol	0.51±0.02*	0.80±0.16*
MDA-MB231	Untreated controls	1.85±0.34	0.48±0.12
	30 μM resveratrol	1.80±0.32	0.41±0.02
HBL100	Untreated controls	0.95±0.04	0.90±0.09
	30 μM resveratrol	1.15±0.18	1.00±0.03

BRCA1 and BRCA2 proteins were obtained by DNA-binding protein purification, specific immunoprecipitation with anti-BRCA1 (556445) or anti-BRCA2 (C-19) antibodies and protein A affinity chromatography and expressed in percentage calculated as follows: 100 × d.p.m. DNA-binding proteins bound specifically to antibodies raised against BRCA1 or BRCA2/d.p.m. of total DNA-binding proteins purified on heparin affinity chromatography. All data are expressed as mean±s.e. of three assays (^*), P<0.05, vs untreated controls (Student's t-test).