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. 2003 Jul 15;89(2):412–419. doi: 10.1038/sj.bjc.6601108

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Copyright © 2003 Cancer Research UK

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4-chloro-ph enylacetae and RAG are synergistic in inhibiting the growth of LA-N-5 cells. Cells were cultured in the presence of 4-CPA (3 mM), RAG (1 μM), 4-CPA (3 mM)+RAG (1 μM), or in the absence of added compounds as indicated. After 5 days, relative proliferation of the cultures was assessed by SRB protein staining. Columns represent the mean (±s.e.m.) of four independent experiments and are expressed as percent of control cultures as assessed by the SRB protein assay. ^* Indicates a significant difference between the single treatment as compared to the control. ^** Indicates significance between combination treatment and treatments with either 4-CPA or RAG alone. Student's t-test (two-tailed) was used for all statistical determinations, where a level of P<0.05 was considered statistically significant.