Table 2. Oligonucleotide primer and probe sequences for real-time quantitative RT-PCR.
| Templates | Oligonucleotides | Sequences | PCR product size (bp) |
|---|---|---|---|
| EGFR | Forward primer | 5′-TCCCTCAGCCACCCATATGTAC-3′ | 125 |
| Reverse primer | 5′-GTCTCGGGCCATTTTGGAGAATCC-3′ | ||
| Probe | 5′-afATCACCbQCACGGAACTTTGGGCGACTATCTGCGTC-3′ | ||
| LRIG1 | Forward primer | 5′-GGTGAGCCTGGCCTTATGTGAATA-3′ | 108 |
| Reverse primer | 5′-CACCACCATCCTGCACCTCC-3′ | ||
| Probe | 5′-afACACATbQGGTAGCGGCCCTCGTGCCCG-3′ | ||
| 18S | Forward primer | 5′-CGGCGACGACCCATTCGAAC-3′ | 99 |
| Reverse primer | 5′-GAATCGAACCCTGATTCCCCGTC-3′ | ||
| Probe | 5′-afCCTATCAACbQTTCGATGGTAGTCGCCGTGCC-3′ |
a
Note. f denotes a 5′-conjugated fluorescein and
b
Q denotes a T with a conjugated Dark Quencher molecule.