Figure 2.

CD26/DPPIV-associated enhancement in PARP cleavage induced by topoisomerase II inhibitors. CD26 Jurkat transfectants were incubated at 37°C with media containing etoposide for 16 h or doxorubicin for 18 h at the indicated doses. Cells were then harvested, and whole-cell lysates were obtained. Following SDS–PAGE of lysates, immunoblotting studies for PARP and β-actin were performed as described in Materials and Methods. The cleaved product of PARP was detected at ∼85 kDa. Each lane was loaded with 30 μg of protein.