Figure 4.

Effect of caspase-9 inhibitor z-LEHD-fmk on etoposide-induced apoptosis in wtCD26 Jurkat transfectant. wtCD26 Jurkat transfectants were incubated at 37°C for 2 h of preincubation with z-LEHD-fmk at varying doses, and then treated with 3 μM etoposide or 1 μM doxorubicin for 16 h. Cells were then harvested, and whole-cell lysates were obtained as described in Materials and Methods. Following SDS–PAGE of lysates, immunoblotting studies for PARP, caspase-3, caspase-9, and β-actin were performed as described in Materials and Methods (^*): caspase-3 cleaved products. Each lane was loaded with 30 μg of protein.