Figure 6.

Effect of soluble CD26 molecules on topoisomerase II alpha expression. Parental Jurkat cells (A) or Jiyoye cells (B) were incubated overnight in culture media alone (−) or culture media containing soluble CD26 (sCD26) molecules (300 μg ml⁻¹) (+) at 37°C. Cells were then harvested and nuclear extracts were obtained. Following SDS–PAGE of lysates, immunoblotting studies for topoisomerase II alpha or β-actin were performed as described in Materials and Methods. Each lane was loaded with 30 μg of protein.