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. 2003 Oct 14;89(8):1581–1589. doi: 10.1038/sj.bjc.6601300

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Copyright © 2003 Cancer Research UK

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Cellular distribution of BODIPY-taxol in TS^Adr. TS^Adr (d=300–400 μM) were incubated with 2.5 μM BODIPY-taxol for 60 min at 37°C in the presence or absence of 10 μM nicardipine. Images were produced by optical sectioning to a depth of 80 μM at 4 μM increments along the z-axis of the tissue. The fluorescence intensity was measured for each of the images along the xy-axis. (A, D and G) intensity measured at depths of 20, 40 or 80 μM from the periphery along the z-axis. (B, E and H) images taken in TS^Adr at depths of 20, 40 or 80 μM along the z-axis (intensities are shown in the graphs as a dotted line). (C, F and I) images obtained from TS^Adr that had been incubated with 10 μM nicardipine prior to addition of BODIPY-taxol (intensities shown in the graphs as solid lines).