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. 2003 Oct 14;89(8):1508–1512. doi: 10.1038/sj.bjc.6601324

Table 1. PCR primers and probes.

GenBank accession: NM_003118 SPARC
Forward primer: SPARC
Sequence: 5′-TCTTCCCTGTACACTGGCAGTTC-3′
Reverse primer: SPARC
Sequence: 5′-AGCTCGGTGTGGGAGAGGTA-3′
TaqMan probe: SPARC
Sequence: 6FAM 5′-CAGCTGGACCAGCACCCCATTGAC-3′TAMRA
GenBank accession: BC016045 β-actin
Forward primer: β-actin
Sequence: 5′-TGAGCGCGGCTAC AGCTT-3′
Reverse primer: β-actin
Sequence: 5′-TCCTTAATGTCACGCACGATTT-3′
TaqMan probe: β-actin
Sequence: 6FAM5′-ACCACCACGGCC GAGCGG-3′TAMRA