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. 2003 Oct 14;89(8):1566–1573. doi: 10.1038/sj.bjc.6601317

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Copyright © 2003 Cancer Research UK

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Time-dependent induction of p53 and p21^WAFI/CIP1 expression in androgen-dependent (AD) and- independent (AI) prostate cancer cell lines by vinorelbine. Androgen-independent and AI cells grown exponentially were exposed to 0.1 μM of vinorelbine for indicated periods of time. The cells were harvested, washed, and total proteins extracted. Proteins (50 μg) were subjected to 4/10% stacking SDS–PAGE, electrotransferred to nitrocellulose membrane, and immunoblotted with p21^WAFI/CIP1 and p53 monoclonal antibodies, respectively. The same membrane was stripped, and reprobed with antibody against β-actin for equal loading control.