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. 2003 Oct 14;89(8):1566–1573. doi: 10.1038/sj.bjc.6601317

Figure 3.

Figure 3

Effects of paclitaxel (left panel) and vinorelbine (middle and right panel) on the expression of p53 and p21WAFI/CIPI in androgen-dependent (AD, left and middle panel) and-independent (AI, right panel) prostate cancer cell lines. AD and AI cells grown exponentially were exposed for 24 h to different concentrations of paclitaxel (left panel) or vinorelbine (middle and right). The cells were harvested, washed, and total proteins extracted. Fifty μg of proteins were subjected to 4%/10% stacking SDS-PAGE, electrotransferred to nitrocellulose membrane, and immunoblotted with p21WAFI/CIPI and p53 monoclonal antibodies, respectively. The same membrane was stripped and reprobed using antibody against β-actin for equal loading control.