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. 2003 Oct 14;89(8):1566–1573. doi: 10.1038/sj.bjc.6601317

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Copyright © 2003 Cancer Research UK

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Effect of vinorelbine (NVB) or paclitaxel (TAX) on p21 transactivation in AD or AI cells. Androgen-dependent or AI cells grown at exponential phase were cotransfected in serum-free RPMI-1640 medium with 1 μg cDNA of full promoter-p21 reporter and 1 μg cDNA of pSV-β-galactosidase using Effectene according to manufacturer's instructions. At 1 day after the transfection, the cells were exposed for 24 h to concentrations of NVB or TAX, as indicated. The cells were washed, and lysed for luciferase activity assay as described in Materials and Methods. The luciferase activities in AD or AI cells were normalised to the activity of β-galactosidase for the equal efficiency of the transfections. The data are expressed as means±s.d. from three separate experiments.