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. 2003 Oct 14;89(8):1566–1573. doi: 10.1038/sj.bjc.6601317

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Copyright © 2003 Cancer Research UK

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Role of p53 binding sites within the promoter of p21 gene played in the vinorelbine-mediated p21 activation in AD and AI cells. Androgen-dependent and AI cells grown at exponential phase were cotransfected in serum-free RPMI-1640 medium with 1 μg cDNA of full promoter-p21 reporter or pWPdel-BstXI and 1 μg cDNA of pSV-β-galactosidase using Effectene according to the manufacturer's instructions. At 1 day after the transfection, the cells were exposed for 24 h to concentrations of vinorelbine as indicated. The cells were washed and lysed for luciferase activity assay as described in Materials and Methods. The luciferase activities in AD and AI cells were normalised to the activity of β-galactosidase for the equal efficiency of the transfections. The data are expressed as means± s.d. from three separate experiments.