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. 2003 Oct 14;89(8):1566–1573. doi: 10.1038/sj.bjc.6601317

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Copyright © 2003 Cancer Research UK

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Involvement of Spl-3 and Spl-4 within the promoter of p21 gene in the vinorelbine-mediated p21 transcriptional activation in androgen-independent prostate cancer cells. Androgen-independent cells grown at exponential phase were cotransfected in serum-free RPMI-1640 medium with 1 μg cDNA of full promoter p21 reporter, pWPdel-BstXI, pWPlOl, pWPdel-Sma I, Spl-p21, or mtSpl-p21 and 1 μg cDNA of pSV-β-galactosidase using Effectene according to the manufacturer's instructions. At 1 day after the transfection, the cells were exposed for 24 h to different concentrations of vinorelbine, as indicated. The cells were washed, and lysed for luciferase activity assay as described in Materials and Methods. The luciferase activities in AD and AI cells were normalised to the activity of β-galactosidase for the equal efficiency of the transfections. The data are expressed as means±s.d. from three separate experiments.